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JoVE Journal
Immunology and Infection

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Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes
 

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

Article DOI: 10.3791/61562-v 09:20 min November 12th, 2020
November 12th, 2020

Chapters

Summary

Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages.

Tags

Keywords: Monocyte Differentiation Macrophage Cord Blood Stem Cell Exosomes Immune Modulation CD14+ Cells Cell Separation Ultracentrifugation Stem Cell Educator Therapy
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