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Flow Cytometric Characterization of Murine B Cell Development
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Characterization of Murine B Cell Development

Flow Cytometric Characterization of Murine B Cell Development

DOI:
10.3791/61565-v

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08:25 min

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January 22, 2021

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, , , , , , , , ,
1Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Chapters

  • 00:04Introduction
  • 00:55Stain Cells and Prepare Compensation
  • 05:30Flow Cytometric Data Acquisition and Analysis
  • 06:08Results: Flow Cytometric Analysis for the Characterization of B Cells
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.

Tags

Keywords: Flow CytometryB Cell DevelopmentMurine ModelsAutoimmunityAntibody TherapeuticsFluorophoresPhenotypingGenetic ManipulationBone MarrowSpleenViability DyeSingle Color Compensation

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