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Microscopic Observation of Lymphocyte Dynamics in Rat Peyer’s Patches
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Microscopic Observation of Lymphocyte Dynamics in Rat Peyer’s Patches

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer’s Patches

DOI:
10.3791/61568-v

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06:31 min

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June 25, 2020

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, , , , ,
1Department of internal medicine,National Defense Medical College, 2Division of Gastroenterology and Hepatology, Department of Internal Medicine,Jikei University School of Medicine

Chapters

  • 00:04Introduction
  • 00:42Collection and Separation of Lymphocytes
  • 03:12Lymphocyte Labeling and Experimental Setup for Microvascular Studies
  • 04:40Microcirculation of Lymphocytes
  • 05:25Results: Microscopic Images of Peyer s Patches from Rats
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.

Tags

Lymphocyte DynamicsPeyer’s PatchesIn Vivo ObservationRatCannulationThoracic DuctAbdominal SurgeryAnesthesiaHeparinDuodenumInfusionBollman’s Cage

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