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鼠佩耶斑块中淋巴细胞动力学的微观观察
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Medicine
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JoVE Journal
Medicine
Microscopic Observation of Lymphocyte Dynamics in Rat Peyer’s Patches
Please note that all translations are automatically generated.
Click here for the English version.
鼠佩耶斑块中淋巴细胞动力学的微观观察
DOI:
10.3791/61568-v
•
06:31 min
•
June 25, 2020
•
Kazuhiko Shirakabe
2
,
Masaaki Higashiyama
,
Naoki Shibuya
,
Kazuki Horiuchi
,
Masayuki Saruta
,
Ryota Hokari
1
Department of internal medicine
,
National Defense Medical College
,
2
Division of Gastroenterology and Hepatology, Department of Internal Medicine
,
Jikei University School of Medicine
Chapters
00:04
Introduction
00:42
Collection and Separation of Lymphocytes
03:12
Lymphocyte Labeling and Experimental Setup for Microvascular Studies
04:40
Microcirculation of Lymphocytes
05:25
Results: Microscopic Images of Peyer s Patches from Rats
05:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个精确的方法,收集胸管淋巴细胞和观察肠道热带淋巴细胞在老鼠Peyer的补丁的迁移3小时使用延时摄影。这项技术可以澄清淋巴细胞的动态在炎症条件下是如何受到影响的。
Tags
Lymphocyte Dynamics
Peyer’s Patches
In Vivo Observation
Rat
Cannulation
Thoracic Duct
Abdominal Surgery
Anesthesia
Heparin
Duodenum
Infusion
Bollman’s Cage
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