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1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research,Università degli Studi di Milano, 2Department of Veterinary Medicine,University of Sassari, 3Department of Agricultural and Environmental Sciences – Production, Landscape, Agroenergy and Center for Stem Cell Research,Università degli Studi di Milano
Chapters
- 00:04Introduction
- 01:11Fibroblast Exposure to 5-aza-CR
- 02:28Fibroblast Encapsulation in PTFE Micro-Bioreactors
- 04:05Culture in PTFE Micro-Bioreactors of Epigenetically Erased Cells
- 05:00Results: Epigenetically Erased Cells Encapsulated in PTFE Micro-Bioreactors Show High Level and Long-term Maintenance of Pluripotency-Related Gene Expression
- 07:33Conclusion
Vi presenterer her en metode som kombinerer bruk av kjemisk epigenetisk sletting med mechanosensing-relaterte signaler for effektivt å generere pattedyr pluripotente celler, uten behov for gentransfeksjon eller retrovirale vektorer. Denne strategien er derfor lovende for translasjonsmedisin og representerer et bemerkelsesverdig fremskritt innen stamcelleorganoidteknologi.
