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在伤口反应期间斑马鱼中性粒细胞中趋化因子受体的实时成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses
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在伤口反应期间斑马鱼中性粒细胞中趋化因子受体的实时成像

DOI:
10.3791/61679-v

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06:48 min

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December 04, 2020

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, , , ,
1Department of Physiology, Development and Neuroscience,University of Cambridge

Chapters

  • 00:04Introduction
  • 01:04Larval Ventral Fin Wounding and Live Imaging Preparation
  • 02:22Live Confocal Imaging
  • 03:03Zebrafish Neutrophil Receptor Internalization Quantification
  • 04:36Results: Representative Differential CXCR1 and CXCR2 Wound Response Dynamics
  • 06:12Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了对斑马鱼嗜中性粒细胞的化学引诱剂受体动力学进行实时成像和定量分析的方案。

Tags

ZebrafishNeutrophilsWound ResponseChemokine ReceptorsLive ImagingIn VivoInflammationImmune ResponseTransgenicFin WoundingConfocal MicroscopyTime-lapse ImagingReceptor Internalization

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