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Journal
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Biology
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中子大分子晶体学晶体生长优化
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Optimization of Crystal Growth for Neutron Macromolecular Crystallography
Please note that all translations are automatically generated.
Click here for the English version.
中子大分子晶体学晶体生长优化
DOI:
10.3791/61685-v
•
12:29 min
•
March 13, 2021
•
Elham Vahdatahar
,
Niels Junius
2
,
Monika Budayova – Spano
1
CEA, CNRS, IBS
,
Université Grenoble Alpes
,
2
ELVESYS SAS
Chapters
00:00
Introduction
01:49
Protocol: Instrument/ Flow-cell
03:59
Protocol: Software
07:33
Results
11:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
通过晶体学对生物分子进行结构研究需要高质量的晶体。在这里，我们演示了 OptiCrys（在我们的实验室中开发的全自动仪器）和/或微透析按钮可用于基于结晶相图知识种植大型高质量晶体的协议。
Tags
Keywords: Crystal Growth
Neutron Macromolecular Crystallography
Dialysis Method
Temperature-controlled Dialysis Crystallization
Micro Dialysis Buttons
Lysozyme
Nucleation
Metastable Zone
OptiCrys
Phase Diagram
Protein Crystallization
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