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1Laboratory of Molecular Gerontology, National Institute on Aging,National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology,Hunan University, 3Department of Molecular Biology and Genetics,Cornell University, 4Department of Cellular and Molecular Medicine,University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
Chapters
- 00:04Introduction
- 00:51Cell Preparation
- 03:58Proximity Ligation Assay (PLA)
- 06:20Results: Representative Replisome Encounter Visualization
- 07:52Conclusion
Enquanto colisões de bifurcação de replicação com adutos de DNA podem induzir quebras de fita dupla, pouco se sabe sobre a interação entre replisomos e lesões de bloqueio. Empregamos o ensaio de ligadura de proximidade para visualizar esses encontros e caracterizar as consequências para a composição do replisome.
