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样品室光片荧光显微镜中多个昆虫胚胎的同步实时成像

JoVE Journal
Biology

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JoVE Journal Biology

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

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样品室光片荧光显微镜中多个昆虫胚胎的同步实时成像

DOI:

10.3791/61713-v

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08:29 min

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September 09, 2020

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Julia Ratke*¹, Franziska Krämer*¹, Frederic Strobl

¹Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF – MC),Goethe-Universität

Chapters

00:04Introduction
00:39Light Sheet Fluorescence Microscope Calibration
02:37Sodium Hypochlorite-Based Dechorionation
03:48Embryo Mounting
04:51Comparative Live Imaging
06:02Imaged Embryo Retrieval and Cultivation
06:52Results: Representative Drosophila and Tribolium Fluorescence Live Imaging
07:56Conclusion

Summary

Automatic Translation

光片荧光显微镜是发育生物学中最有价值的工具。比较研究中的一个主要问题是环境差异。我们的协议描述了多个标本同时进行实时成像的实验框架，因此，主动解决这个问题。

Tags

Keywords: Light Sheet Fluorescence Microscopy Sample Chamber Cobweb Holder Fluorescent Microspheres Embryo Dechorionation Simultaneous Live Imaging High Content Imaging Calibration Axial Resolution

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