Journal
/
Biology
/
Samtidig levende bildebehandling av flere insektembryoer i prøvekammerbaserte lysarkfluorescensmikroskoper
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Samtidig levende bildebehandling av flere insektembryoer i prøvekammerbaserte lysarkfluorescensmikroskoper

DOI:
10.3791/61713-v

•

08:29 min

•

September 09, 2020

•

, ,
1Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF – MC),Goethe-Universität

Chapters

  • 00:04Introduction
  • 00:39Light Sheet Fluorescence Microscope Calibration
  • 02:37Sodium Hypochlorite-Based Dechorionation
  • 03:48Embryo Mounting
  • 04:51Comparative Live Imaging
  • 06:02Imaged Embryo Retrieval and Cultivation
  • 06:52Results: Representative Drosophila and Tribolium Fluorescence Live Imaging
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Lysarkbasert fluorescensmikroskopi er det mest verdifulle verktøyet i utviklingsbiologi. Et stort problem i komparative studier er omgivelsesvariasjon. Vår protokoll beskriver et eksperimentelt rammeverk for samtidig levende bildebehandling av flere prøver og tar derfor opp dette problemet proaktivt.

Tags

Keywords: Light Sheet Fluorescence MicroscopySample ChamberCobweb HolderFluorescent MicrospheresEmbryo DechorionationSimultaneous Live ImagingHigh Content ImagingCalibrationAxial Resolution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61713?article_type=v

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
check_url/61713?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/61713?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/61713?article_type=v

Imaging Cell Shape Change in Living Drosophila Embryos
check_url/61713?article_type=v

Overvåking plasmidreplikasjon i Live pattedyrceller over flere generasjoner av fluorescensmikropskopi
check_url/61713?article_type=v

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
check_url/61713?article_type=v

Multilayer Montering for Long-term Lett Sheet Mikroskopi av Sebrafisk
check_url/61713?article_type=v

Sette opp et enkelt Lett Sheet mikroskop for<em> I Toto</em> Imaging av<em> C. elegans</em> Utvikling
check_url/61713?article_type=v

Semi-automatisert Imaging av Tissue-spesifikk fluorescens i sebrafisk embryo
check_url/61713?article_type=v

In Vivo 4-Dimensional Sporing av Hematopoietiske Stem og stamceller i Adult Mouse Calvarial Bone Marrow

Read Article