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一种富含病毒的接种物用于蜜蜂口腔感染（Apis mellifera）的制备
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JoVE Journal Biology
Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)
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一种富含病毒的接种物用于蜜蜂口腔感染（Apis mellifera）的制备

DOI:
10.3791/61725-v

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06:41 min

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August 26, 2020

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, ,
1Department of Entomology,University of Illinois, 2Department of Microbiology,Center for Scientific Research and Higher Education of Ensenada (CICESE)

Chapters

  • 00:04Introduction
  • 00:41Larval Self-Removal
  • 02:08Pupal Virus Injection
  • 03:19Viral Feeding Bioassay
  • 04:58Results: Representative Virus Load and Survival Curve Analyses
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了两种方案：第一种是繁殖，提取，纯化和量化大量蜜蜂非包膜病毒颗粒，包括去除蜜蜂蛹的方法，其次是使用高度可重复的高通量笼式生物测定来测试病毒感染的影响。

Tags

Keywords: Virus-enriched InoculumOral InfectionHoney BeesApis MelliferaVirus ParticlesBioassaysVirus TreatmentBee PupaeVirus ProductionPollinator SystemsVirus InfectivityMass Bee ExtractionHoney Bee QueenLarval Self-removalLangstroth FrameIncubatorPBSVirus InjectionManual Multipipette

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