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Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
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Bioengineering
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JoVE Journal Bioengineering
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

DOI:
10.3791/61735-v

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14:48 min

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April 17, 2021

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, , , ,
1State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology,Northwest University

Chapters

  • 00:00Introduction
  • 02:23Microfluidic Chip Design
  • 04:33Chip Fabrication and Operation
  • 05:32Microfluidic Chip Production using Soft Lithograph
  • 07:03Chip Operation
  • 07:59Generation of Dynamic Inputs in Cellular Microenviroments
  • 08:43Setup for High Throughput Live-cell Imaging
  • 10:44Automatic Data Analysis
  • 11:42Discussion

Summary

Automatic Translation

Automatic Translation

We present a microfluidic system for high throughput studies on complex life machinery, which consists of 1500 culture units, an array of enhanced peristaltic pumps and an on-site mixing modulus. The microfluidic chip allows for the analysis of the highly complex and dynamic micro-environmental conditions in vivo.

Tags

Microfluidic DeviceHigh-throughputDynamic Environmental ConditionsCell CulturePeristaltic PumpShear-free CultureStem Cell DifferentiationOrgan-on-chip

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