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非编码小RNA MicC有助于肠炎 沙门氏菌 外膜蛋白的毒力
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
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非编码小RNA MicC有助于肠炎 沙门氏菌 外膜蛋白的毒力

DOI:
10.3791/61808-v

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06:30 min

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January 27, 2021

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, , , , ,
1College of Veterinary Medicine,Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Department of Animal Husbandry and Veterinary Medicine,Beijing Agricultural Vocational College

Chapters

  • 00:04Introduction
  • 00:40Construction of the MicC Deletion Mutant
  • 04:11Results: PCR Verification and Virulence Properties of the 50336△micC Mutants
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

使用λ-Red介导的重组系统创建小的非编码RNA micC的缺失突变体。

Tags

Homologous RecombinationGene InactivationVirulence GenesAttenuated MutantSalmonella EnteritidisChloramphenicol CassetteMicC Gene

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