/

Developmental Biology

/

卵子微弹射和有效交配的基因组编辑在火布拉特 热比亚家

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Please note that all translations are automatically generated. Click here for the English version.

卵子微弹射和有效交配的基因组编辑在火布拉特热比亚家

DOI:

10.3791/61885-v

•

06:08 min

•

October 20, 2020

•

Takahiro Ohde, Toshinori Minemura, Eiichi Hirose, Takaaki Daimon

¹Department of Applied Biosciences, Graduate School of Agriculture,Kyoto University

Chapters

00:04Introduction
00:41Egg Collection and Microinjection
03:04Mating
03:42Genotyping
04:31Results: Dry Injection of T. domestica Eggs
05:37Conclusion

Summary

Automatic Translation

我们为卵子的饲养、微喷射和在基因组编辑后生成和维持突变菌株的火布拉特 热比亚家用菌 株的有效交配提供了详细的协议。

Tags

Keywords: Thermobia Domestica Firebrat Egg Microinjection Genome Editing Insect Model Culture Maintenance RNAi Transgenesis Laboratory Model

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

技术目标显微注射到显影<em>爪</em>肾

临界条件在免疫染色豌豆蚜虫胚胎鉴定：增加组织通透性和降低背景染色

显微注射转基因的基因组，并在编辑Threespine刺鱼

在成像钙<em>果蝇</em>在蛋激活

胚胎显微注射和电的脊索动物<em>玻璃海鞘</em

雌性生殖道交配后精子的收集及对小鼠精子液化的测定

基于基因组编辑的果蝇组织特异二进制转录系统的高效生成策略

基于微注射的免疫胚胎心肌细胞体内植入系统

基因组编辑细胞系细胞融合，用于细胞结构和功能的扰动

Read Article