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Egg Microinjection og effektiv parring for Genom Redigering i Firebrat Thermobia domestica
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
DOI:

06:08 min

October 20, 2020

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Chapters

  • 00:04Introduction
  • 00:41Egg Collection and Microinjection
  • 03:04Mating
  • 03:42Genotyping
  • 04:31Results: Dry Injection of T. domestica Eggs
  • 05:37Conclusion

Summary

Automatic Translation

Vi leverer en detaljeret protokol for opdræt, mikroinjection af æg og for effektiv parring af firebrat Thermobia domestica at generere og vedligeholde mutant stammer efter genom redigering.

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