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纳索尼亚维特里彭尼斯的 Rnai 和克里斯普基因编辑的普帕尔和成人注射
JoVE Journal
Biology
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JoVE Journal Biology
Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis
DOI:

08:41 min

December 04, 2020

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Chapters

  • 00:04Introduction
  • 01:04Nasonia Rearing
  • 02:19Alignment of White and Black Pupae
  • 03:21Needle Preparation
  • 03:53Adult Injection with Aspirator Tube
  • 05:04Adult Injection with Aspirator Tube
  • 06:32Post-Injection Care and Mutant Screening
  • 07:27Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections
  • 07:52Conclusion

Summary

Automatic Translation

在这里,我们将 纳索尼亚维特里彭尼斯 高效注射 pupal 和成人注射的方法描述为胚胎微注射的可访问替代品,通过 RNA 干扰 (RNAi) 或通过 CRISPR/Cas9 基因组编辑进行基因敲除,实现感兴趣的基因的功能分析。

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