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Journal
/
Behavior
/
测量
果蝇
拉瓦的机械结节的改进的检测和工具
JoVE Journal
Behavior
This content is Free Access.
JoVE Journal
Behavior
An Improved Assay and Tools for Measuring Mechanical Nociception in
Drosophila
Larvae
Please note that all translations are automatically generated.
Click here for the English version.
测量
果蝇
拉瓦的机械结节的改进的检测和工具
DOI:
10.3791/61911-v
•
07:17 min
•
October 29, 2020
•
Roger Lopez-Bellido
,
Michael J. Galko
2,3
1
Department of Genetics
,
The University of Texas MD Anderson Cancer Center
,
2
Neuroscience Graduate Program, Graduate School of Biomedical Sciences
,
The University of Texas MD Anderson Cancer Center
,
3
Genetics and Epigenetics Graduate Program, Graduate School of Biomedical Sciences
,
The University of Texas MD Anderson Cancer Center
Chapters
00:04
Introduction
00:56
Mechanical Probe Construction
02:26
Preparation of Larvae
03:16
Mechanical Nociception Assay
04:33
Results: Representative Mechanical Nociception and Hypersensitivity
06:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议的目的是展示如何对
嗜血杆菌
幼虫的机械结节进行改进的检测。我们在这里使用测定来证明机械过敏(合金和高过敏症)存在于
嗜血杆菌
幼虫中。
Tags
Mechanical Nociception
Drosophila Larvae
Custom Tools
Nitinol Filaments
Dose-response Curves
Probe Calibration
Force Measurement
Pressure Conversion
Larval Sorting
Third Instar Stage
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