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将小鼠大脑显微切割成功能和解剖学上不同的区域
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
DOI:

08:06 min

February 15, 2021

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Chapters

  • 00:04Introduction
  • 01:41Mouse Brain Removal
  • 03:26Mouse Brain Dissection
  • 06:40Results: Mouse Brain Removal and Dissection
  • 07:22Conclusion

Summary

Automatic Translation

我们提出了一个动手的,一步一步的,快速的方案,用于从新鲜脑组织中去除小鼠大脑和解剖离散区域。获取用于分子分析的大脑区域已成为许多神经科学实验室的常规。这些大脑区域立即被冷冻,以获得高质量的转录组学数据,用于系统级分析。

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