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睡眠美人转座转染人视网膜色素上皮细胞氧化应激的诱导与分析
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JoVE Journal Medicine
Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
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睡眠美人转座转染人视网膜色素上皮细胞氧化应激的诱导与分析

DOI:
10.3791/61957-v

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10:00 min

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December 11, 2020

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, , , , ,
1Experimental Ophthalmology,University of Geneva, 2Department of Ophthalmology,University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine

Chapters

  • 00:04Introduction
  • 00:52Conditioned Medium Preparation and Collection
  • 02:47Treatment of RPE Cells with Growth Factors and H2O2
  • 04:33Glutathione Assay
  • 06:00Cytotoxicity Assay and Microscopic Analysis
  • 07:24Results: Representative Oxidative Stress Induction and Analysis
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一种开发和使用氧化应激模型的方案，方法是用H2O2处理视网膜色素上皮细胞，分析细胞形态，活力，密度，谷胱甘肽和UCP-2水平。它是研究转座子转染细胞分泌的蛋白质治疗神经视网膜变性的抗氧化作用的有用模型。

Tags

Oxidative StressHydrogen PeroxideSleeping Beauty TransposonHuman Retinal Pigment Epithelial CellsAge-related Macular DegenerationNeurodegenerative DiseasesARPE-19 Cell LinePEDFGM-CSFNickel NTAProtein PurificationBCA Protein AssayConditioned MediumHydrogen Peroxide Treatment

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