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呼吸系统同步病毒病毒特定核胶囊的生成和组装

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

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呼吸系统同步病毒病毒特定核胶囊的生成和组装

DOI:

10.3791/62010-v

•

09:08 min

•

July 27, 2021

•

Yunrong Gao, Claire Ogilvie, Anirudh Raghavan, Chloe Von Hoffmann, Bo Liang

¹Department of Biochemistry,Emory University School of Medicine

Chapters

00:04Introduction
01:10Protein Expression and Purification
04:12In Vitro Virus-Specific Nucleocapsid (NC) Assembly
05:19Negative Stain Grid Preparation
07:17Results: Representative N1-391P1-126 Complex Copurification
08:08Conclusion

Summary

Automatic Translation

为了深入分析呼吸道同步病毒（RSV） RNA 合成，我们报告了利用伴生磷蛋白（P）共同表达无RNA核蛋白（N^0）的协议，用于病毒特定核胶囊（NCs）的后续体外组装。

Tags

Keywords: Virus-specific Nucleocapsids Respiratory Syncytial Virus Recombinant Viral Protein Expression RNA-free N Protein Ligation Independent Cloning Electron Microscopy Bi-cistronic Construction Protein Expression Chromatography Cobalt Gravity Column Q Column HPLC UV Detection

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