Journal
/
Medicine
/
Laser Doppler Perfusion Imaging in the Mouse Hindlimb
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Laser Doppler Perfusion Imaging in the Mouse Hindlimb

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

DOI:
10.3791/62012-v

•

14:45 min

•

April 18, 2021

•

,
1Division of Vascular Surgery,University of Washington, 2VA Puget Sound Health Care System

Chapters

  • 00:00Introduction
  • 02:39Scanner Preparation
  • 03:14Mouse Pre-Scanning Preparation
  • 06:44Scanning
  • 09:52Capturing LDPI Data
  • 11:22Representative Results
  • 12:18Troubleshooting
  • 14:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that demonstrates the technique and necessary controls for Laser Doppler perfusion imaging to measure blood flow in the mouse hindlimb.

Tags

Laser Doppler Perfusion ImagingLDPIMouse HindlimbSkin UlcerationWound HealingPeripheral Arterial DiseaseRheumatologic ConditionsDiabetesBlood FlowIschemiaIschemia ReperfusionMicrosurgical FlapsAnesthetic AgentsSkin PigmentationArteriogenesisAngiogenesisCollateral Artery DevelopmentMicrofill CastingMicro CT

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62012?article_type=v

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
check_url/62012?article_type=v

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
check_url/62012?article_type=v

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat
check_url/62012?article_type=v

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
check_url/62012?article_type=v

Technical Aspects of the Mouse Aortocaval Fistula
check_url/62012?article_type=v

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
check_url/62012?article_type=v

Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion
check_url/62012?article_type=v

Imaging Metals in Brain Tissue by Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS)
check_url/62012?article_type=v

Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo
check_url/62012?article_type=v

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Read Article