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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
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Neuroscience
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JoVE Journal Neuroscience
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

DOI:
10.3791/62016-v

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11:42 min

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June 10, 2021

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, , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

Chapters

  • 00:00Introduction
  • 01:01Direct Conversion of Human Fibroblasts to Neuronal Progenitor Cells
  • 04:21Conversion and iNPC Splitting
  • 06:42Generating Induced Astrocytes From NPCs
  • 07:43Astrocyte Differentiation From Frozen iNPC Stocks
  • 08:51Results: Growth and Characterization of Cells Throughout Conversion Process
  • 10:53Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

Tags

Keywords: In Vitro ModelingNeurological DiseasesFibroblastsNeuronal Progenitor CellsAstrocytesDirect ConversionSkin BiopsyRetroviral VectorsOct3/4Klf4Sox2C-MycTransductionNeurodegenerative DiseasesCell CultureCo-culturesDisease MechanismsMedication Testing

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