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贝克酵母糖精中线粒体基因组表达产品的标记和可视化
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Biochemistry
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JoVE Journal Biochemistry
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae
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贝克酵母糖精中线粒体基因组表达产品的标记和可视化

DOI:
10.3791/62020-v

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08:33 min

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April 11, 2021

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, , , , ,
1Department of Biology,M.V. Lomonosov Moscow State University, 2Institute of Functional Genomics,M.V. Lomonosov Moscow State University, 3National Research Centre “Kurchatov Institute”

Chapters

  • 00:00Introduction
  • 00:39Preparation of Yeast Culture
  • 02:10Radioactive Isotope Incorporation
  • 03:34Yeast Cell Lysis and Extraction of Proteins
  • 04:20Precipitation of Proteins
  • 05:15Electrophoretic Separation
  • 05:43Autoradiography
  • 05:59Representative Results
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

贝克的酵母线粒体基因组编码了八种多肽。当前协议的目标是将所有这些都标记为单独的频段，然后将其可视化为单独的频段。

Tags

Mitochondrial GenomeMitochondrial Protein BiosynthesisRadioactive LabelingGel ElectrophoresisSaccharomyces CerevisiaeYeastTranslationCycloheximideS35 MethionineBorymycin

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