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Biochemistry
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荧光泄漏测定研究细胞穿透肽对膜的破坏稳定性
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Biochemistry
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Biochemistry
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
Please note that all translations are automatically generated.
Click here for the English version.
荧光泄漏测定研究细胞穿透肽对膜的破坏稳定性
DOI:
10.3791/62028-v
•
07:33 min
•
December 19, 2020
•
Karidia Konate
,
Quentin Seisel
,
Eric Vivès
,
Prisca Boisguérin
,
Sébastien Deshayes
1
PHYMEDEXP
,
INSERM U1046 – CNRS UMR 9214 – University of Montpellier
Chapters
00:05
Introduction
00:53
Large Unilamellar Vesicle (LUV) Preparation
02:57
LUV Purification
03:51
LUV Size and Homogeneity Characterization
04:21
Fluorescence Leakage Assay
05:20
Results: Representative Fluorescence Leakage Analysis
06:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
荧光泄漏测定是一种简单的方法,可以研究肽/膜相互作用,以了解它们在几个生物过程中的参与,特别是细胞穿透肽在直接细胞易位过程中扰乱磷脂双层的能力。
Tags
Keywords: Fluorescence Leakage Assay
Membrane Destabilization
Cell-penetrating Peptides
Liposomes
Lipid Composition
Large Unilamellar Vesicles (LUVs)
Phosphatidylcholine
Sphingomyelin
Cholesterol
Lipid Hydration
Freeze-thaw
Extrusion
Size Exclusion Chromatography
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