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Creation and Maintenance of a Living Biobank – How We Do It
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Creation and Maintenance of a Living Biobank – How We Do It

Creation and Maintenance of a Living Biobank – How We Do It

DOI:
10.3791/62065-v

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13:08 min

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April 10, 2021

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, , , , ,
1Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, University of Rostock, 2Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, 3Institute of Pathology,University Medical Center Rostock

Chapters

  • 00:00Introduction
  • 00:36Sample Acquisition
  • 02:41Tissue Processing
  • 05:43Cell Culture
  • 07:40Generation of Patient-Derived Xenografts
  • 11:16Explantation of PDX
  • 11:44Representative Results
  • 12:41Conclusion

Summary

Automatic Translation

Automatic Translation

In the following work, we describe the consecutive steps necessary for the establishment of a large biobank of colorectal and pancreatic cancer.

Tags

Keywords: Living BiobankPatient-derived ModelsPrecision MedicineSample AcquisitionSerum ProcessingPBL IsolationSnap Freezing

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