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1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Chapters
- 00:05Introduction
- 00:29Design of Gene-Specific Primers and Preparation of sgRNA and Embryos
- 02:18Microinjection of Embryos
- 03:47Post-Injection Insect Rearing
- 04:41Knock-Out Mutation Detection
- 05:27Results: Selection and Synthesis of Target sgRNAs and Detection of Mutants by PCR and Gel Electrophoresis
- 06:08Conclusion
Burada CRISPR/Cas9 genom düzenlemesi tarafından oluşturulan Helicoverpa armigera (Hübner) embriyo mikroenjeksiyonu ve nakavt mutant tanımlama protokolü sunulmaktadır. Mutant böcekler, in vivo'daki farklı genler arasında gen fonksiyonu ve etkileşiminin daha fazla araştırılmasına olanak tanır.
