Journal
/
Bioengineering
/
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DOI:
10.3791/62073-v

•

09:26 min

•

December 29, 2021

•

, ,
1Institute of Biomedical Engineering,University of Toronto

Chapters

  • 00:04Introduction
  • 00:32Oligonucleotide to Small Nuclear RNA-Activating Protein (SNAP) T7 RNA Polymerase (RNAP) Conjugation and Polyacrylamide Gel (PAGE) Analysis
  • 02:04Oligonucleotide-Tethered SNAP-T7 Purification
  • 04:13On-Demand Control Tethered RNAP Activity Demonstration
  • 07:08Results: Representative DNA-Tethered T7 RNAP Purification and Analysis
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the engineering of a novel DNA-tethered T7 RNA polymerase to regulate in vitro transcription reactions. We discuss the steps for protein synthesis and characterization, validate proof-of-concept transcriptional regulation, and discuss its applications in molecular computing, diagnostics, and molecular information processing.

Tags

Keywords: DNA-tethered RNA PolymeraseProgrammable In Vitro TranscriptionMolecular ComputationGene Regulatory ArchitectureDNA-based CircuitsTranscriptional CircuitsSNAP T7 RNAPOligonucleotide-tethered SNAP T7 PurificationIon Exchange ColumnElution Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62073?article_type=v

Adaptation of a Haptic Robot in a 3T fMRI
check_url/62073?article_type=v

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
check_url/62073?article_type=v

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
check_url/62073?article_type=v

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
check_url/62073?article_type=v

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
check_url/62073?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/62073?article_type=v

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
check_url/62073?article_type=v

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
check_url/62073?article_type=v

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
check_url/62073?article_type=v

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Read Article