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Chapters
- 00:05Introduction
- 00:42Generation of 3D Collagen Dermal Component
- 01:44Cell Laden Collagen Seeding of Dermal Compartment
- 03:16Seeding of Epidermal Component and Stratification Induction
- 04:53Immunofluorescent Staining of 3D Constructs
- 05:46Prepare Imaging Wells
- 07:14Results: Characterization of in Vitro Vascularized Human Skin Equivalents (VHSE)
- 08:22Conclusion
The goal of this protocol is to describe the generation and volumetric analysis of vascularized human skin equivalents using accessible and simple techniques for long term culture. To the extent possible, the rationale for steps is described to allow researchers the ability to customize based on their research needs.
