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JoVE Journal
Neuroscience

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Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
 

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

Article DOI: 10.3791/62126-v 07:40 min February 11th, 2021
February 11th, 2021

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Summary

This paper describes methods for tissue preparation, staining, and analysis of whole fungiform, circumvallate, and palate taste buds that consistently yield whole and intact taste buds (including the nerve fibers that innervate them) and maintain the relationships between structures within taste buds and the surrounding papilla.

Tags

Keywords: Whole-mount Staining Taste Buds Fungiform Circumvallate Palate Lingual Epithelium Innervation Transducing Cells Tissue Dissection Tissue Freezing OCT Compound
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