A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:49Collection of Pupae for Microscopic Characterization and Identification of Fluorescent Markers
- 01:45Sexing Pupae
- 02:25Setting Up GAL4-UAS (Upstream Activation Sequences) System Crosses and Inducing Egg Laying
- 03:20Embryo Fixation and Bleaching
- 04:15Results: Analysis of Anopheles gambiae Pupae Expressing Fluorescent Markers Driven by 3xP3 Promoter, Tissue-specific Expression Visualization, and Embryo Clearing
- 05:53Conclusion
