Journal
/
Developmental Biology
/
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

DOI:
10.3791/62163-v

•

06:56 min

•

January 21, 2021

•

, , ,
1Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences,University of Georgia

Chapters

  • 00:04Introduction
  • 00:37Separation of the Tongue Epithelium from the Mesenchyme/Underlying Connective Tissue
  • 03:51Cell Dissociation
  • 05:06Results: Effect of Cell Dissociation on Total Cell Number and Viability
  • 06:29Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.

Tags

Keywords: Cell DissociationTongue EpitheliumTongue MesenchymeEmbryonic Day 12.58-week-old MiceSingle Cell RNA SequencingPrimary Stem Cell CulturesEnzymatic DigestionTyrode’s SolutionDispaseCollagenase

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62163?article_type=v

Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells
check_url/62163?article_type=v

Assaying Blood Cell Populations of the Drosophila melanogaster Larva
check_url/62163?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/62163?article_type=v

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
check_url/62163?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/62163?article_type=v

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
check_url/62163?article_type=v

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
check_url/62163?article_type=v

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
check_url/62163?article_type=v

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
check_url/62163?article_type=v

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Read Article