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在体内成像完全活跃的脑组织在清醒斑马鱼幼虫和青少年由骷髅和皮肤去除

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Please note that all translations are automatically generated. Click here for the English version.

在体内成像完全活跃的脑组织在清醒斑马鱼幼虫和青少年由骷髅和皮肤去除

DOI:

10.3791/62166-v

•

05:25 min

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February 10, 2021

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Paul Schramm, Florian Hetsch, Jochen C. Meier, Reinhard W. Köster

¹Cellular and Molecular Neurobiology, Zoological Institute,Technische Universität Braunschweig, ²Institute of Pathophysiology,University Medical Center of the Johannes Gutenberg University, ³Cell Physiology, Zoological institute,Technische Universität Braunschweig

Chapters

00:04Introduction
00:57Larva Preparation
01:39Larva Embedding
02:22Brain Exposure
03:21Results: Representative In Vivo Fully Active Awake Zebrafish Larva and Juvenile Brain Tissue Imaging
04:26Conclusion

Summary

Automatic Translation

在这里，我们提出了一种方法，以图像斑马鱼胚胎大脑在体内到幼虫和幼年阶段。这种微创程序，根据电生理学方法改编，提供成熟神经元的细胞和亚细胞细节，并可与光遗传学和神经药理学研究相结合，以描述大脑功能和药物干预。

Tags

Keywords: In Vivo Imaging Zebrafish Larvae Zebrafish Juveniles Skull Removal Skin Removal Neuronal Architecture Pigment Cells Larval Development Synaptic Plasticity Neuron Degeneration Neuron Regeneration ACSF Agarose Embedding Anesthesia Glass Needle Skin Incision Brain Exposure

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