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Immunology and Infection

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在囊性纤维化肺中的伪肌瘤和金黄色葡萄球菌生物膜的前体内模型中进行抗生素疗效测试

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Please note that all translations are automatically generated. Click here for the English version.

在囊性纤维化肺中的伪肌瘤和金黄色葡萄球菌生物膜的前体内模型中进行抗生素疗效测试

DOI:

10.3791/62187-v

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09:26 min

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January 22, 2021

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Niamh E. Harrington*¹, Esther Sweeney*¹, Ilyas Alav², Freya Allen, John Moat³, Freya Harrison

¹School of Life Science,University of Warwick, ²Institute of Microbiology and Infection,University of Birmingham, ³Warwick Antimicrobial Screening Facility, School of Life Science,University of Warwick

Chapters

00:04Introduction
00:52Dissection and Infection of Ex Vivo Pig Lung (EVPL) Tissue
04:45Determination of Antibiotic Efficacy
06:39Results: Screening Bacterial Isolates for Antibiotic Susceptibility with the EVPL Model
08:49Conclusion

Summary

Automatic Translation

此工作流程可用于使用囊性纤维化个体肺部细菌生物膜的既定前活体模型进行抗生素易感性测试。使用此模型可以提高MBEC（最低生物膜根除浓度）检测的临床有效性。

Tags

Keywords: Antibiotic Efficacy Pseudomonas Aeruginosa Staphylococcus Aureus Biofilms Cystic Fibrosis Lung Ex Vivo Model Bronchiole DMEM RPMI 1640 Antibiotic Susceptibility Testing

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