JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
内源性表达人RYR1变异体的功能表征
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Functional Characterization of Endogenously Expressed Human RYR1 Variants
Please note that all translations are automatically generated.
Click here for the English version.
内源性表达人RYR1变异体的功能表征
DOI:
10.3791/62196-v
•
07:59 min
•
June 09, 2021
•
Susan Treves
2
,
Thierry Girard
,
Francesco Zorzato
2
1
Department of Biomedicine
,
Basel University Hospital
,
2
Department of Life Sciences
,
University of Ferrara
,
3
Department of Anesthesia
,
Basel University Hospital
Chapters
00:04
Introduction
01:00
Intracellular Cell Culture Ca
2+
Measurement
02:30
Intracellular Single Cell Ca
2+
Measurement
03:56
Human Myotube Preparation
04:40
Human Myotube Inducible Calcium ([Ca
2+
]
i
) Measurement
05:33
Results: Representative Single Cell Ca
2+
Release in EBV-Immortalized Human B Cells and Isolated Human Myotubes
07:11
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这里描述了用于研究RYR1突变的功能效应的方法,这些突变在爱泼斯坦巴尔病毒中内源性表达,使人B淋巴细胞永生化,肌肉活检衍生的卫星细胞分化成肌管。
Tags
RYR1 Variants
Calcium Homeostasis
EBV-transformed B Lymphocytes
Fura-2 AM
Krebs Ringer’s Solution
Calcium Release Response
Single Cell Analysis
Spectrofluorometer
Perfusion Chamber
Fluorescent Microscopy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
利用人类纤维原细胞线粒体表型的监测领域的帕金森氏病
人牙髓干细胞的分离,鉴定和比较性恒牙使用两种不同的方法,得出
隔离与人类心室肌细胞的功能研究从新鲜手术样品
使用功能性磁共振成像连接在颞叶癫痫默认模式网络的网络分析
在
小鼠大肠传入结尾的
体外
功能研究
功能人类肝脏保存和恢复由Subnormothermic机灌注手段
后皮肤微移植生成一个新的方法解聚表征的组织
纵<em>在体内</em>的Cerebrovasculature影像:相关性疾病CNS
应用流式细胞仪对人体脂肪组织免疫细胞的表征
小鼠致耐受性树突状细胞的发育及功能表征
Read Article