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Journal
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Immunology and Infection
/
观察活胰腺组织切片中的胰岛功能和胰岛-免疫细胞相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices
Please note that all translations are automatically generated.
Click here for the English version.
观察活胰腺组织切片中的胰岛功能和胰岛-免疫细胞相互作用
DOI:
10.3791/62207-v
•
05:51 min
•
April 12, 2021
•
Mollie K. Huber
,
Denise M. Drotar
3,4
,
Helmut Hiller
,
Maria L. Beery
,
Paul Joseph
,
Irina Kusmartseva
,
Stephan Speier
3,4
,
Mark A. Atkinson
,
Clayton E. Mathews
,
Edward A. Phelps
1
Department of Pathology, Immunology, and Laboratory Medicine
,
University of Florida
,
2
Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden
,
3
Institute of Physiology, Faculty of Medicine
,
Technische Universität Dresden
,
4
German Center for Diabetes Research (DZD)
,
5
J. Crayton Pruitt Family Department of Biomedical Engineering
,
University of Florida
Chapters
00:05
Introduction
00:37
Generation of the Mouse Pancreatic Slices
02:18
Immune Cell Recordings on Mouse Pancreatic Tissue Slices
03:59
Results: Viability and Immune Cell Activity of the Live Pancreatic Tissue Slices
05:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
本研究介绍了活胰腺组织切片在胰岛生理学和胰岛免疫细胞相互作用研究中的应用。
Tags
Keywords: Islet Function
Islet-immune Cell Interactions
Pancreatic Illnesses
Pancreatitis
Type I Diabetes
Type II Diabetes
Vibratome
Extracellular Solution
Krebs-Ringer Bicarbonate Buffer
Trypsin Inhibitor
Slice Culture Medium
Microscope
Brightfield Mode
Islets
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