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Enkelt Myofiber Kultur Assay til vurdering af voksne muskelstamcellefunktionalitet Ex Vivo
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
DOI:

09:19 min

February 15, 2021

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Chapters

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

Automatic Translation

I denne protokol beskrives en in vitro-dyrkningsmetode og funktionel analysemetode for muskelstamceller, som bevarer de fleste af deres interaktioner med deres endogene niche.

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