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Chapters
- 00:04Introduction
- 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
- 01:54EDL Muscle Isolation and Collagenase Digestion
- 03:19Myofiber Dissociation and Culture
- 04:20siRNA Transfection, Fixation, and IF Staining
- 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
- 08:34Conclusion
