/

/

评估人体骨骼肌微组织骨骼肌健康的功能指标

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Please note that all translations are automatically generated. Click here for the English version.

评估人体骨骼肌微组织骨骼肌健康的功能指标

DOI:

10.3791/62307-v

•

09:30 min

•

February 18, 2021

•

Heta Lad², Brennen Musgrave², Majid Ebrahimi², Penney M. Gilbert^2,3

¹Institute of Biomedical Engineering,University of Toronto, ²Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ³Department of Cell and Systems Biology,University of Toronto

Chapters

00:04Introduction
00:42Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
02:51Preparation of Cells for hMMTs Seeding
05:24Seeding hMMTs
07:24Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
08:57Conclusion

Summary

Automatic Translation

本手稿描述了一种详细的方案，用于生成3D人骨骼肌微系统和微创下游功能原位测定阵列，包括收缩力和钙处理分析。

Tags

Muscle Biology Disease Modeling Candidate Molecule Testing Contractile Force Calcium Transients Longitudinal Studies Basement Membrane Extract Thrombin Fibrinogen

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

工程骨骼肌组织中的小鼠成肌细胞的内皮祖细胞与应用电刺激

在肌肉再生的老鼠模型中移植诱导多能干细胞衍生的中生代细胞样肌原体

最大等长收缩力的测量通过透骨骼肌纤维生成

工程化血管肌瓣

计算机断层扫描和成骨血管生成耦合光学成像评估头盖骨自体移植和移植物的整合

中的应用<em>在体内</em>评定组织的鼠胫骨前的功能测试工程化骨骼肌修复

心肌肌细胞基致动器和自稳定Biorobot - 第1部分

心肌肌细胞基致动器和自稳定Biorobot - 第2部分

半自动纵 Microcomputed Tomography-based 裸鼠骨质疏松症相关椎体骨折模型的定量结构分析

用于组织工程应用的三维聚 (ε-内) 支架的熔体静电纺丝

Read Article