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Vurdering af funktionelle målinger af skeletmuskulatursundhed i humane skeletmuskulaturmikrotisser
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JoVE Journal Bioengineering
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Vurdering af funktionelle målinger af skeletmuskulatursundhed i humane skeletmuskulaturmikrotisser

DOI:
10.3791/62307-v

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09:30 min

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February 18, 2021

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, , ,
1Institute of Biomedical Engineering,University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 3Department of Cell and Systems Biology,University of Toronto

Chapters

  • 00:04Introduction
  • 00:42Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
  • 02:51Preparation of Cells for hMMTs Seeding
  • 05:24Seeding hMMTs
  • 07:24Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver en detaljeret protokol til fremstilling af arrays af 3D menneskelige skeletmuskulaturmikrotiss og minimalt invasive downstream in situ-analyser af funktion, herunder kontraktilkraft og calciumhåndteringsanalyser.

Tags

Muscle BiologyDisease ModelingCandidate Molecule TestingContractile ForceCalcium TransientsLongitudinal StudiesBasement Membrane ExtractThrombinFibrinogen

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