Journal
/
Biochemistry
/
Microcrystal Electron Diffraction of Small Molecules
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Microcrystal Electron Diffraction of Small Molecules

Microcrystal Electron Diffraction of Small Molecules

DOI:
10.3791/62313-v

•

09:48 min

•

March 15, 2021

•

,
1Howard Hughes Medical Institute,University of California Los Angeles, 2Department of Biological Chemistry,University of California Los Angeles, 3Department of Physiology,University of California Los Angeles

Chapters

  • 00:04Introduction
  • 00:34Preparing Small Molecule Samples and TEM Grids
  • 01:37Applying Sample to Grids by Creating a Homogenous Fine Powder
  • 02:51Freezing and Loading Grids into the TEM
  • 04:12Locating and Screening Nanocrystals
  • 07:33Collecting MicroED Data
  • 08:36Results: Analysis of MicroED Dataset from Carbamazepine Microcrystal
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the procedures developed in our laboratory for preparing powders of small molecule crystals for microcrystal electron diffraction (MicroED) experiments.

Tags

Keywords: Microcrystal Electron DiffractionSmall MoleculesPowderNanometer Size CrystalsAtomic Resolution StructuresTEM GridsGlow DischargeFilter PaperPowder TransferLiquid Nitrogen

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62313?article_type=v

Microcrystallography of Protein Crystals and In Cellulo Diffraction
check_url/62313?article_type=v

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
check_url/62313?article_type=v

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
check_url/62313?article_type=v

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
check_url/62313?article_type=v

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy
check_url/62313?article_type=v

Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
check_url/62313?article_type=v

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
check_url/62313?article_type=v

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
check_url/62313?article_type=v

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
check_url/62313?article_type=v

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Read Article