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Biochemistry
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小分子的微晶电子衍射
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Microcrystal Electron Diffraction of Small Molecules
Please note that all translations are automatically generated.
Click here for the English version.
小分子的微晶电子衍射
DOI:
10.3791/62313-v
•
09:48 min
•
March 15, 2021
•
Michael W. Martynowycz
2
,
Tamir Gonen
2,3
1
Howard Hughes Medical Institute
,
University of California Los Angeles
,
2
Department of Biological Chemistry
,
University of California Los Angeles
,
3
Department of Physiology
,
University of California Los Angeles
Chapters
00:04
Introduction
00:34
Preparing Small Molecule Samples and TEM Grids
01:37
Applying Sample to Grids by Creating a Homogenous Fine Powder
02:51
Freezing and Loading Grids into the TEM
04:12
Locating and Screening Nanocrystals
07:33
Collecting MicroED Data
08:36
Results: Analysis of MicroED Dataset from Carbamazepine Microcrystal
09:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了我们实验室开发的用于制备用于微晶电子衍射(MicroED)实验的小分子晶体粉末的程序。
Tags
Microcrystal Electron Diffraction
Small Molecules
Powder
Nanometer Size Crystals
Atomic Resolution Structures
TEM Grids
Glow Discharge
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Powder Transfer
Liquid Nitrogen
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