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中性粒细胞中活性氧物种的实时定量 感染脑膜性 大肠杆菌
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
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中性粒细胞中活性氧物种的实时定量 感染脑膜性 大肠杆菌

DOI:
10.3791/62314-v

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11:00 min

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April 20, 2021

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, ,
1Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology,Ministry of Education, China Medical University

Chapters

  • 00:00Introduction
  • 00:50Preparation of the Bacteria Strain
  • 02:21Isolation of Neutrophils
  • 07:41Measurement of ROS
  • 09:51Representative Results
  • 12:22Conclusion

Summary

Automatic Translation

Automatic Translation

大肠杆菌 是新生儿革兰阴性细菌性脑膜炎的主要原因。在细菌感染期间，中性粒细胞产生的活性氧物种起着主要的杀菌作用。在这里，我们介绍了一种检测中性粒细胞中反应性氧物种以应对 大肠杆菌脑膜炎的方法。

Tags

Reactive Oxygen SpeciesNeutrophilsEscherichia ColiMeningitisNeonatal Bacterial MeningitisBacteremiaBlood-brain BarrierHost DefenseROS ProductionBacterial ColonyBrain-Heart Infusion MediumRifampicinPeripheral Blood SamplesRed Blood Cell LysisMagnetic Cell SortingWhite Blood CellsNeutrophils

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