Journal
/
Immunology and Infection
/
Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

DOI:
10.3791/62317-v

•

13:00 min

•

July 31, 2021

•

, , ,
1Division of Laboratory Animal Resources,Duke University Medical Center, 2Department of Pathology,Duke University Medical Center, 3Department of Immunology,Duke University Medical Center, 4Duke Human Vaccine Institute,Duke University Medical Center

Chapters

  • 00:04Introduction
  • 00:43Maternal Intranasal Immunization Study Design
  • 03:19Intranasal Immunization
  • 06:03Droplet Issues
  • 06:33Milk Collection
  • 07:49Milk Collection Demonstration
  • 10:07Representative Results
  • 12:17Summary

Summary

Automatic Translation

Automatic Translation

This article describes and demonstrates the administration of intranasal vaccines and the collection of milk from lactating rabbits (Oryctolagus cuniculus) as a means to assess mucosal immunity in a translationally appropriate model of maternal immunization.

Tags

Keywords: Maternal ImmunizationRabbit ModelIntranasal ImmunizationMilk CollectionAntibody TransferTransplacental Antibody TransferIgGIgANew Zealand White RabbitsPlacentationMaternal-fetal ImmunityNewborn ProtectionPassive Immunity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62317?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/62317?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/62317?article_type=v

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
check_url/62317?article_type=v

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
check_url/62317?article_type=v

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
check_url/62317?article_type=v

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
check_url/62317?article_type=v

A Method for Targeted 16S Sequencing of Human Milk Samples
check_url/62317?article_type=v

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
check_url/62317?article_type=v

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
check_url/62317?article_type=v

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Read Article