/
/
Generering av en forenklet tredimensjonal hud-på-en-chip-modell i en mikromaskin mikrofluidisk plattform
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Bioengineering and Aerospace Engineering,Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology,Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine,CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón
Chapters
- 00:04Introduction
- 00:48Chip Design and Micromachining Parameters
- 01:44Chip Assembly
- 02:43Pump Connections and Parallel Flow Protocol
- 03:55Human Immortalized Skin Keratinocytes (hKCs) Monolayer Seeding
- 04:31Results: Characterization of Skin Model in The Microfluidic Chip with Confocal and Fluorescence Microscopy
- 05:49Conclusion
Her presenterer vi en protokoll for å generere en tredimensjonal forenklet og uavklart hudmodell ved hjelp av en mikromaskinert mikrofluidisk plattform. En parallell strømningstilnærming tillater in situ-avsetning av et dermalt rom for såing av epitelceller på toppen, alt kontrollert av sprøytepumper.
