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Biology
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细胞扩散过程中细胞边缘动力学的定量分析
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Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
Please note that all translations are automatically generated.
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细胞扩散过程中细胞边缘动力学的定量分析
DOI:
10.3791/62369-v
•
10:54 min
•
May 22, 2021
•
Ernest Iu*
1
,
Alexander Bogatch*
1
,
Sergey V. Plotnikov
1
Department of Cell and Systems Biology
,
University of Toronto
Chapters
00:00
Introduction
01:03
Coverslip Preparation and Cell Seeding
03:42
Drug Incubation and Cell Recovery
06:18
Magnetic Chamber Preparation
07:29
Image Acquisition
08:26
Computer Analysis of Spreading Dynamics
09:38
Representative Results
10:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在此协议中,我们介绍了基于活细胞显微镜的细胞扩散检测的实验程序。我们为荧光标记细胞的无偏见分割和细胞扩散过程中的跛脚足动力学定量分析提供了开源计算工具。
Tags
Cell Edge Dynamics
Cell Spreading
Quantitative Analysis
Automated Analysis
Cell Morphology
Cell Protrusions
PH-Akt-GFP
Fibronectin Coating
Cell Seeding
Trypsinization
Live-cell Imaging
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