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细胞扩散过程中细胞边缘动力学的定量分析
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
DOI:

10:54 min

May 22, 2021

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Chapters

  • 00:00Introduction
  • 01:03Coverslip Preparation and Cell Seeding
  • 03:42Drug Incubation and Cell Recovery
  • 06:18Magnetic Chamber Preparation
  • 07:29Image Acquisition
  • 08:26Computer Analysis of Spreading Dynamics
  • 09:38Representative Results
  • 10:25Conclusion

Summary

Automatic Translation

在此协议中,我们介绍了基于活细胞显微镜的细胞扩散检测的实验程序。我们为荧光标记细胞的无偏见分割和细胞扩散过程中的跛脚足动力学定量分析提供了开源计算工具。

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