Journal
/
Biology
/
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

DOI:
10.3791/62418-v

•

08:11 min

•

August 07, 2021

•

, ,
1Structural Genomics Consortium,University of Toronto, 2Department of Pharmacology and Toxicology,University of Toronto

Chapters

  • 00:00Introduction
  • 00:47Cell Lysate Preparation
  • 03:06Western Blot Analysis
  • 06:41Representative Results
  • 07:36Conclusions

Summary

Automatic Translation

Automatic Translation

These protocols provide the methodology used to assess the enzymatic activity of individual members of the protein arginine methyltransferase (PRMT) family in cells. Detailed guidelines on assessing PRMT activity using endogenous and exogenous biomarkers, methyl-arginine recognizing antibodies, and inhibitor tool compounds are described.

Tags

Keywords: Protein Arginine MethyltransferaseEnzymatic ActivityQuantitative Fluorescent Western BlottingBiomarkersCell LysisProtein ConcentrationGel ElectrophoresisWestern Blot Transfer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62418?article_type=v

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
check_url/62418?article_type=v

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
check_url/62418?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/62418?article_type=v

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
check_url/62418?article_type=v

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
check_url/62418?article_type=v

In vitro Methylation Assay to Study Protein Arginine Methylation
check_url/62418?article_type=v

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
check_url/62418?article_type=v

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
check_url/62418?article_type=v

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
check_url/62418?article_type=v

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D

Read Article