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视网膜器官诱导系统，用于从人类多能干细胞中提取3D视网膜组织
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JoVE Journal Medicine
Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells
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视网膜器官诱导系统，用于从人类多能干细胞中提取3D视网膜组织

DOI:
10.3791/62435-v

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10:05 min

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April 12, 2021

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, ,
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-sen University, Guangzhou, China, 510060

Chapters

  • 00:04Introduction
  • 01:19Human Pluripotent Stem Cells (hPSCs) Culture
  • 03:48Embryoid Body (EB) Formation
  • 05:56Seed the Embryoid Bodies (EBs)
  • 07:00Detach and Culture OVs and RPE in Suspension to Obtain Retinal Organoids (ROs)
  • 07:45Results: Analysis of Morphological Features of ROs and Subtypes of Retinal Cells from hPSCs
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个优化的视网膜器官诱导系统，它适用于各种人类多能干细胞系，以产生具有高可重复性和效率的视网膜组织。

Tags

Retinal OrganoidHuman Pluripotent Stem CellsRetinal InductionRetinal DevelopmentDisease ModelingCell TherapyRetinal Degenerative DiseasesEmbryoid BodyPlating DensityRetinal CellsEDTA Dissociation

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