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Retinal organoid induksjonssystem for avledning av 3D retinal vev fra humane pluripotente stamceller
JoVE Journal
Medicine
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JoVE Journal Medicine
Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells
DOI:

10:05 min

April 12, 2021

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Chapters

  • 00:04Introduction
  • 01:19Human Pluripotent Stem Cells (hPSCs) Culture
  • 03:48Embryoid Body (EB) Formation
  • 05:56Seed the Embryoid Bodies (EBs)
  • 07:00Detach and Culture OVs and RPE in Suspension to Obtain Retinal Organoids (ROs)
  • 07:45Results: Analysis of Morphological Features of ROs and Subtypes of Retinal Cells from hPSCs
  • 09:39Conclusion

Summary

Automatic Translation

Her beskriver vi et optimalisert retinal organoid induksjonssystem, som er egnet for ulike menneskelige pluripotente stamcellelinjer for å generere netthinnevev med høy reproduserbarhet og effektivitet.

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