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激光微辐射研究S阶段DNA招募
JoVE Journal
Biology
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JoVE Journal Biology
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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激光微辐射研究S阶段DNA招募

DOI:
10.3791/62466-v

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07:11 min

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April 16, 2021

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1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, 3Howard Hughes Medical Institute,New York University School of Medicine

Chapters

  • 00:04Introduction
  • 00:47Preparation of Cells for Micro-Irradiation
  • 01:18Selecting S Phase Cells for Imaging
  • 02:40Micro-Irradiation for Immunofluorescence Staining or Time Lapse Imaging
  • 03:44Time Lapse Imaging
  • 04:47Results: DNA Recruitment During S Phase After Micro-Irradiation
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种非侵入性方法，用于有效识别下游显微镜研究的S相细胞，例如通过激光微辐射测量DNA修复蛋白的招募。

Tags

Keywords: Laser Micro-irradiationDNA RepairS PhasePCNASpatiotemporal ResolutionDNA Damage Repair Protein KineticsConfocal ImagingOlaparibEnvironmental ChamberMicroscopeMPlumBinary LinesROIND StimulationTime Schedule

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