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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement
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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement

Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement

DOI:
10.3791/62511-v

•

07:53 min

•

August 26, 2021

•

, , ,
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Chapters

  • 00:05Introduction
  • 00:53Cell Embedding in Methylcellulose Hydrogel
  • 03:03Cell Resuspension for Proplatelet Analysis
  • 04:24Cell Fixation and Retrieval for Future Analyses
  • 05:16Results: Megakaryocyte Characteristics and Proplatelet Quantification According to Culture Conditions
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.

Tags

Megakaryocyte3D CultureMethylcellulose HydrogelCell MaturationConfinementStiffnessHematopoietic Stem CellsProplateletsBone Marrow

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